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Identification And Location Of Symbionts Associated With Potato Psyllid (Bactericera cockerelli) Lifestages

Daymon Hail, Scot E. Dowd, Blake Bextine
DOI: http://dx.doi.org/10.1603/EN11198 98-107 First published online: 1 February 2012


The potato psyllid (Bactericera cockerelli, Sulc) is an invasive pest of solenaceous plants including potatoes (Solanum tuberosum L.)and tomatoes (Solanum lycopersicum L.). The insect transmits the phytopathogen Candidatus Liberibacter solanacearum, which has been identified as the causal agent of Zebra Chip in potatoes. The microbiome of the potato psyllid provides knowledge of the insect's bacterial makeup which enables researchers to develop targeted biological control strategies. In this study, the microbes associated with four B. cockerelli life stages were evaluated by 16S bTEFAP pyrosequencing. The sequences were compared with a 16S-rDNA database derived from NCBI's GenBank. Some bacteria identified are initial discoveries. Species of Wolbachia, Rhizobium, Gordonia, Mycobacterium, Xanthomonas and others were also detected and an assessment of the microbiome associated with B. cockerelli was established.

  • pyrosequencing
  • psyllids
  • microbiome
  • symbionts
  • massively parallel bacterial tag-encoded FLX-Titanium amplicon pyrosequencing
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